PREPARATION OF TEMPORARY SPECIMENS AND STUDY OF CELL ORGANOIDS USING THEM
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Abstract
The preparation of temporary (wet mount) specimens is a fundamental technique in cell biology and histology, allowing researchers and students to observe cell structures in their near-natural state. This method provides rapid visualization of cellular components, organelles, and their dynamic interactions without extensive sample processing. The present study focuses on the principles and procedures of temporary specimen preparation, emphasizing the preservation of cell morphology and organoid structures. Various staining techniques, mounting media, and microscopy methods are discussed to enhance the contrast and resolution of subcellular organoids. The study also highlights the educational and experimental applications of temporary preparations, demonstrating their role in understanding cellular organization, physiological processes, and pathological changes. Finally, the advantages and limitations of this technique are evaluated, providing guidelines for effective observation and analysis of organoids in vitro.
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