BIOMEDICAL APPLICATIONS OF SOY ISOLATE AND SERICIN-BASED THREE-COMPONENT FILM MATERIALS: THEIR EFFECTIVENESS IN EMULSIFICATION AND SKIN REGENERATION

Abstract

This article provides an in-depth review of the biomedical applications of three-component film materials based on soy protein isolate (SPI) and silk sericin (SS), specifically analyzing their effectiveness in emulsification processes (stabilization of water and oil phases) and skin regeneration (fibroblast proliferation, collagen synthesis, and epithelialization). It examines in detail the synergistic properties of natural polymers, including the amphipathic nature, essential amino acids, and bioadhesiveness of SPI, the antioxidant, anti-inflammatory, and hydrophilic properties of SS, and the role of third components (e.g., carboxymethylcellulose - CMC, polyvinyl alcohol - PVA, bacterial cellulose - BC) in optimizing mechanical strength and biodegradation. The synthesis methods (solution casting, solvent casting, electrospinning), properties (mechanical strength, water absorption, thermal stability) and advantages in biomedical applications of the materials, including controlled drug release, accelerated wound healing and stability in emulsion formulations, are discussed. Based on in vitro (proliferation and migration experiments in L929 and NIH-3T3 cells) and in vivo (full-thickness wounds in rat models) studies, these films are confirmed to be highly effective in diabetic wounds, burns and other complex conditions by inhibiting inflammation, stimulating angiogenesis and reducing scar formation. The environmental friendliness of the materials (obtained from natural sources, non-toxicity, low allergic reactions) and their advantages over synthetic analogues are also emphasized. The article provides suggestions for future nanotechnological integrations (nanofibrillar structures, microspheres), clinical trials, and cosmetic-pharmaceutical applications (anti-aging agents, pigmentation control), which will contribute to opening new directions in biomedical engineering. Overall, the research demonstrates the potential for providing environmentally and economically sustainable medical solutions through the development of natural biomaterials.