MICROBIOLOGICAL ANALYSIS OF ROOT CANAL INFECTIONS AND METHODS FOR THEIR ELIMINATION
DOI:
https://doi.org/10.37547/Keywords:
root canal infection, microbiological analysis, Enterococcus faecalis, sodium hypochlorite, chlorhexidine.Abstract
Root canal infections are among the most common causes of endodontic treatment failure. These infections are primarily polymicrobial, dominated by anaerobic bacteria such as Enterococcus faecalis, Fusobacterium nucleatum, and Prevotella intermedia. The persistence of these microorganisms in the apical and lateral canal systems makes eradication challenging. This study aims to analyze the microbiological spectrum of root canal infections and evaluate effective disinfection strategies. Samples were collected from infected canals using sterile paper points and cultured under aerobic and anaerobic conditions. Molecular methods such as PCR were used for bacterial identification. Results revealed that E. faecalis was the most resistant species, particularly in previously treated teeth. Disinfection using sodium hypochlorite (NaOCl), chlorhexidine, and laser-assisted irrigation demonstrated significant bacterial reduction. Combination protocols using NaOCl and EDTA followed by photodynamic therapy yielded the highest sterilization rates. This research highlights the necessity of integrating microbiological diagnostics into clinical endodontics and adopting multi-step disinfection protocols to prevent reinfection and improve long-term treatment success.
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References
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